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Senthil, N.
- Profiling of Metabolites from Human Intervertebral Disc through Gas Chromatography - Mass Spectrometry
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Authors
R. Veera Ranjani
1,
N. Senthil
1,
M. Raveendran
1,
S. Vellaikumar
1,
R. Gnanam
1,
M. K. Rishi
2,
S. Rajasekaran
2
Affiliations
1 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultutal University, Coimbatore - 641003, Tamil Nadu, IN
2 Department of Orthopaedics and Spine Surgery, Ganga Hospital, Coimbatore, Tamil Nadu, IN
1 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultutal University, Coimbatore - 641003, Tamil Nadu, IN
2 Department of Orthopaedics and Spine Surgery, Ganga Hospital, Coimbatore, Tamil Nadu, IN
Source
Indian Journal of Science and Technology, Vol 7, No 8 (2014), Pagination: 1228-1235Abstract
This work aims to identify the metabolites present in the human Intervertebral Disc (IVD). Metabolomic analysis of human IVD tissue has not been extensively done to date. Knowledge on the metabolites present in the IVD tissue in humans is very limited and many compounds are yet to be identified. In this study, we have carried out the metabolic profiling for human IVD through Gas Chromatography/Mass Spectrometry (GC/MS). This is the first initial study that has compared the metabolites of control and diseased IVD. We have identified 75 different chemical compounds in IVD, and also metabolites that are unique to the diseased IVD, suggesting that some of these metabolites might play a role in disc degenerative disease.Keywords
DDD, Human, Intervertebral Disc, Metabolite ProfilingMetabolite Profiling- Evaluation of BC3F1 Progenies against Sorghum Downy Mildew in Maize (Peronosclerospora sorghi)
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Authors
Affiliations
1 Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN
2 Agriculture Research Station (T.N.A.U.), Bhavanisagar (T.N.), IN
3 Department of Biotechnology, Agriculture College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
1 Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN
2 Agriculture Research Station (T.N.A.U.), Bhavanisagar (T.N.), IN
3 Department of Biotechnology, Agriculture College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 228-232Abstract
An experiment was carried out during Rabi, 2013 at Eastern Block of the Central Farm Unit, Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India to identify resistant progenies in BC3F1 population against sorghum downy mildew (SDM) incited by Peronosclerospora sorghi. Sorghum downy mildewis one of the most serious diseases in maize producing areas throughout the world. P. sorghi (SDM) is a factor that limits maize production in several countries of Asia (Rifin, 1983). Therefore, there is a need to develop the new maize cultivars with resistance to SDM in order to enhance the yield. In this present study, experiments were undertaken under vigorous artificial infection conditions in spreader row technique during Rabi, 2013 for characterization of responses of 22 back cross progenies to the SDM; in which 16 progenies were confirmed as phenotypically resistant to sorghum downy mildewviz., UMI 79/936- C1-3-2, UMI 79/936-C1-3-4, UMI 79/936-C1-7-2, UMI 79/936-C1-7-7, UMI 79/936-C1-29-8, UMI 79/936-C1-29-9, UMI 79/ 936-C1-29-13, UMI 79/936-C1-29-23, UMI 79/936-C1-29-35, UMI 79/936-C1-29-36, UMI 79/936-C1-67-3, UMI 79/936-C1-67-12, UMI 79/936-C1-67-25, UMI 79/936-C1-101-12, UMI 79/936-C1-101-13 and UMI 79/936-C1-101-14. Resistant lines will serve as basis material for developing single cross and double cross hybrids for resistance against sorghum downy mildew in maize.Keywords
Maize, Sorghum Downy Mildew, Screening, Back Cross Progenies.References
- Cardwell, K.F., Bock, C., Akinnioye, O.F., Onukwa, D., Adenle, V. and Adetoro, A.O. (1994). Improving screening methods for resistance to downy mildew of maize in Nigeria. Pl. Health Manage. Res. Monogr., 22 : 22-25.
- Craig, J., Bockholt, A.J., Frederiksen, R.A. and Zuber, M.S. (1977). Reaction of important corn inbred lines to Sclerospora sorghi. Plant Dis. Reptr., 61(7) : 563-564.
- George, M.L.C., Prasanna, B.M., Rathore, R.S., Settee, T.A.S., Kasim, F., Azrai, M., Vasal, S., Balla, O., Hautea, D., Canama, A., Regalado, E., Vargas, M., Khairallah, M., Jeffers, D. and Hoisington, D. (2003). Identification of QTLs conferring resistance to downy mildew of maize in Asia. Theor. Appl. Genet., 107 : 544-551.
- Hoisington, D.A. and Melchinger, A.E. (2005). III.3From theory to practice: Marker-assisted selection in maize. Molecular marker systems in plant breeding and crop improvement, pp. 335-352. Springer-Verlag Berlin Heidelberg, Germany.
- Kashmiri, J.P. (2010). Screening of inbreds and SSR genotyping of the F2 mapping population (UMI79 × UMI 936(w)) for tagging sorghum downy mildew resistance in maize (Zea mays L). M.Sc (Ag.) Thesis, Tamil Nadu Agricultural University, Coimbatore, T.N. (INDIA).
- Krishnappa, M., Naidu, B.S. and Seetharam, A. (1995). Inheritance of host resistance to downy mildew in maize. Crop Improv., 22 : 33-37.
- Lal, S. and Singh, I.S. (1984). Breeding for resistance to downy mildews and stalk rots in maize. Theor. Appl. Genet., 69 : 111-119.
- Nair, S.K., Prasanna, B.M., Garg, A., Rathore, R.S., Setty, T.A. S. and Singh, N.N. (2005). Identification and validation of QTLs conferring resistance to sorghum downy mildew (Peronosclerospora sorghi) and Rajasthan downy mildew (P. heteropogoni) in maize. Theor. Appl. Genet., 107 : 544-551.
- Nair, S.K., Prasanna, B.M., Rathore, R.S., Setty, T.A.S., Kumar, R. and Singh, N.N. (2004). Genetic analysis of resistance to sorghum downy mildew and Rajasthan downy mildew in maize (Zea mays L.). Field Crop Res., 89 : 379-387.
- Odovody, G.N. and Frederiksen, R.A. (1984a). Use of systemic fungicides metalaxyl and fosetyl-A1 for control of sorghum downy mildew in corn and sorghum in south Texas. I: Foliar application. Pl. Dis., 68 : 604-607.
- Odovody, G.N. and Frederiksen, R.A. (1984b). Use of systemic fungicides metalaxyl and fosetyl–A1 for control of sorghum downy mildew in corn and sorghum in south Texas. II: Foliar application. Pl. Dis., 68 : 608-609.
- Rathore, R.S. and Jain, M.L. (2000). Management of maize downy mildew through resistant varieties. In: Proc. Indian Phytopathological society- Golden Jubilee, International conference on integrated plant disease management for sustainable agriculture, pp. 160-161.
- Rathore, R.S. and Siradhana, B. (1987). Estimation of losses caused by Perenosclerospora heteropogoni on Ganga-5 maize hybrid. Phytophylactica, 19 : 119-120.
- Renfro, B.L., Pupipat, U., Singburgudom, N., Choonhawongse, K., Bhat, S.S., Singh, J., Wongsinchaum, B., Sardsud, B. and Shah, S.M. (1979). The corn downy mildew disease research program. Bangkok, THAILAND, ITALY.
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- Setty, T., Kumar, T., Gowda, K., Hattappa, S., Ramaswamy, G. and Prasad, N. (2001). Biochemical changes due to Peronosclerospora sorghi infection in resistant and susceptible maize genotypes. Environ. Ecol., 19 : 751-755.
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- Yen, T.T.O., Rathore, R.S., Setty, T.A.S., Kumar, R., Singh, N.N., Vasal, S.K. and Prasanna, B.M. (2001). Inheritance of resistance to sorghum downy mildew (P. sorghi) and Rajasthan downy mildew (P. heteropogoni) in maize in India. Maize Genet. Coop. Newslett., 75: 48-49.
- FAO (Food and Agricultural Organization) (1992). http//www.fao.org.
- Estimation of Mean Performance in Sorghum Downy Mildew Resistant Back Cross Progenies (BC3F1) of Maize
Abstract Views :226 |
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Authors
Affiliations
1 Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN
2 Agriculture Research Station (T.N.A.U.), Bhavanisagar (T.N.), IN
3 Department of Biotechnology, Agriculture College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
1 Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN
2 Agriculture Research Station (T.N.A.U.), Bhavanisagar (T.N.), IN
3 Department of Biotechnology, Agriculture College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 331-336Abstract
The present investigation was carried out at Department of Millets, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India to identify the best performing sorghum downy mildew resistant progeny for agronomical traits. The objective of this study was to identify the better per se performance of the resistant progeny. Twelve biometrical characters of sixteen SDM resistant progenies viz., UMI 79/936-C1-3-2, UMI 79/936-C1-3-4, UMI 79/936-C1-7-2, UMI 79/936-C1-7-7, UMI 79/936-C1-29-8, UMI 79/936-C1-29-9, UMI 79/936-C1-29-13, UMI 79/936-C1-29-23, UMI 79/936-C1-29-35, UMI 79/936-C1-29-36, UMI 79/936-C1-67-3, UMI 79/936-C1-67-12, UMI 79/936-C1-67-25, UMI 79/936-C1-101-12, UMI 79/936-C1-101-13 and UMI 79/936-C1-101-14 were used for mean performance. Studies revealed that among the progenies, UMI 79/936-C1-7-7 and UMI 79/936-C1-7-2 showed better per se performance for yield contributing characters. These two progenies showed highest mean values than the other progenies. It exhibited more mean values than the parents for the characters viz., cob length, cob diameter, number of rows per cob, number of. kernels per row, cob weight, yield per plant, 100 grain weight. Based on the mean values progenies UMI 79/936-C1-7-7 and UMI 79/936-C1-7-2 confirmed as the best progenies.Keywords
Variability Analysis, Sorghum Downy Mildew Resistant Back Cross Progenies, Maize.References
- Aarthi, P. (2012). Molecular marker assisted backcrossing for introgression of sorghum downy mildew resistance into elite inbred of maize (Zea mays L.) M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University. Coimbatore, T.N. (INDIA).
- Bekele, A. and Rao, T.N. (2014). Estimates of heritability, genetic advance and correlation study for yield and it‘s attributes in maize (Zea mays L.). J. Plant Sci., 2(1): 1-4.
- Jeffers, D., Cordova, H., Vasal, S., Srinivasan, G., Beck, D. and Barandiaran, N. (2000). Status in breeding for resistance to maize diseases at CIMMYT. In: Vasal SK, Gonzalez Ceniceros F, Fan XM (Eds.). Proc. 7th Asian Regional Maize Workshop. PCARRD, Los Baos, Philippines, pp. 257–266.
- Panwar, L.L., Mahawar, R.K. and Narolia, R.S. (2013). Genetic variability and interrelationships among grain yield and yield components in maize. Annals Pl. & Soil Res., 15(1) : 15-18.
- Vashishta, A., Dixit, N., Dipika, N., Sharma, S.K. and Marker, S. (2013). Studies on heritability and genetic advance estimates in maize genotypes. Biosci. Discov., 4(2): 165-168.
- FAO (Food and Agricultural Organization) (1992). http//www.fao.org.
- Genetic Diversity Studies in Soybean [Glycine max (L.) Merrill] based on Morphological Characters
Abstract Views :235 |
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Authors
T. V. Shadakshari
1,
T. Kalaimagal
1,
N. Senthil
2,
M. B. Boranayaka
3,
R. Kambe Gowda
4,
G. Rajesha
5
Affiliations
1 Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, (T.N.), IN
2 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T. N.), IN
3 Centre for Plant Breeding and Genetics, Agricultural College and Research Institute, Madurai (T.N.), IN
4 Department of Genetics and Plant Breeding, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
5 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, (T.N.), IN
1 Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, (T.N.), IN
2 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T. N.), IN
3 Centre for Plant Breeding and Genetics, Agricultural College and Research Institute, Madurai (T.N.), IN
4 Department of Genetics and Plant Breeding, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
5 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, (T.N.), IN
Source
Asian Journal of Bio Science, Vol 6, No 1 (2011), Pagination: 7-11Abstract
From the genetic diversity studies based on 12 morphological characters of 50 soybean genotypes it could be inferred that, the traits seeds per plant and seed yield per plant contributed maximum to the genetic diversity. The clustering pattern revealed that there was no correlation between the geographical diversity and genetic diversity. The clusters IX (JS 90-29 and IC18277) and X (IC93656) were the distant clusters and clusters III (IC 39873 and IC 93751) and VIII (IC 34057 and IC 18736) were the nearest clusters. The clusters IX and I possessed the high mean values for many of the traits studied. With respect to seed yield per plant the clusters IX, VI and I were the superior clusters. The entries viz., JS 90-29 and IC18277 of IX cluster and IC93656 of X cluster can be used in crossing programme for development of good recombinants for seed yield.Keywords
Soybean, Diversity, Cluster, Dendrogram.- Seed Development and Maturation Studies In Barnyard Millet Cv. MDU 1
Abstract Views :164 |
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Authors
Affiliations
1 Department of Seed Science and Technology, Agricultural College and Research Institute, Madurai (T.N.), IN
1 Department of Seed Science and Technology, Agricultural College and Research Institute, Madurai (T.N.), IN
Source
International Journal of Agricultural Sciences, Vol 14, No 2 (2018), Pagination: 362-366Abstract
The present investigation was carried out during 2016-2017 to identify the seed development maturation studies in Barnyard millet cv. MDU 1. The results revealed that seeds attained physiological maturity on 50 days after anthesis as evidenced by the maximum panicle length (30.7 cm), fresh weight (31.2 g), dry weight (23.5g), fresh weight of the seeds (23.4g) and dry weight of the seeds (14.5g). The change of colour of panicle and seed from green to dark brown could be considered as a visible index of maturity. The seed quality parameters viz., germination (51 %), shoot length (9.59 cm), ischolar_main length (18.5 cm) and dry matter (0.025 g/10 seedlings) and vigour index (1175) were also maximum at 50 days after anthesis.Keywords
Barnyard Millet, Seed Development, Physiological Maturity, Anthesis.References
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